Little knowledge exists on the lipid-modulating effect of Rubus occidentalis (RO). The present study investigated the molecular mechanisms of lipid-modulating effects of aqueous extract of RO (ROW) in hepatocarcinoma HepG2 cells. ROW decreased apolipoprotein B100 (ApoB100)/apolipoprotein A-1 (ApoA-1) ratio. ROW increased the expression of LDL-receptor (LDL-R). ROW decreased the gene expression of sterol regulatory element-binding protein 1c (SREBP-1c) as well as fatty acid synthase in a concentration-dependent manner. ROW not only down-regulated gene expression of SREBP-2 and HMG-CoA synthase mRNA expression, but also inhibited HMG-CoA reductase activity in a concentration-dependent manner (IC50 = 240.6 μg/ml). These results indicate that ROW decreases the level of bad cholesterol and increases good cholesterol levels by lowering the gene expression of transcription factors, SREBP-1c and SREBP-2, and by inhibiting the expression and/or activity of their downstream enzymes such as fatty acid synthase and HMG-CoA synthase and reductase.
| Published in | American Journal of BioScience (Volume 2, Issue 2) |
| DOI | 10.11648/j.ajbio.20140202.18 |
| Page(s) | 64-69 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Rubus Occidentalis, Apolipoprotein B100, Apolipoprotein A-1, LDL-Receptor, Sterol Regulatory Element-Binding Protein (SREBP), HMG-CoA Reductase
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APA Style
Young-Sook Moon, Ju-Hee Han, Tae-Bum Lee, Ji-Wung Kwon, Cheol-Hee Choi. (2014). Lipid-Modulating Effects of Aqueous Extract of Rubus Occidentalis in Hepatocarcinoma HepG2 Cells. American Journal of BioScience, 2(2), 64-69. https://doi.org/10.11648/j.ajbio.20140202.18
ACS Style
Young-Sook Moon; Ju-Hee Han; Tae-Bum Lee; Ji-Wung Kwon; Cheol-Hee Choi. Lipid-Modulating Effects of Aqueous Extract of Rubus Occidentalis in Hepatocarcinoma HepG2 Cells. Am. J. BioScience 2014, 2(2), 64-69. doi: 10.11648/j.ajbio.20140202.18
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Download@article{10.11648/j.ajbio.20140202.18,
author = {Young-Sook Moon and Ju-Hee Han and Tae-Bum Lee and Ji-Wung Kwon and Cheol-Hee Choi},
title = {Lipid-Modulating Effects of Aqueous Extract of Rubus Occidentalis in Hepatocarcinoma HepG2 Cells},
journal = {American Journal of BioScience},
volume = {2},
number = {2},
pages = {64-69},
doi = {10.11648/j.ajbio.20140202.18},
url = {https://doi.org/10.11648/j.ajbio.20140202.18},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140202.18},
abstract = {Little knowledge exists on the lipid-modulating effect of Rubus occidentalis (RO). The present study investigated the molecular mechanisms of lipid-modulating effects of aqueous extract of RO (ROW) in hepatocarcinoma HepG2 cells. ROW decreased apolipoprotein B100 (ApoB100)/apolipoprotein A-1 (ApoA-1) ratio. ROW increased the expression of LDL-receptor (LDL-R). ROW decreased the gene expression of sterol regulatory element-binding protein 1c (SREBP-1c) as well as fatty acid synthase in a concentration-dependent manner. ROW not only down-regulated gene expression of SREBP-2 and HMG-CoA synthase mRNA expression, but also inhibited HMG-CoA reductase activity in a concentration-dependent manner (IC50 = 240.6 μg/ml). These results indicate that ROW decreases the level of bad cholesterol and increases good cholesterol levels by lowering the gene expression of transcription factors, SREBP-1c and SREBP-2, and by inhibiting the expression and/or activity of their downstream enzymes such as fatty acid synthase and HMG-CoA synthase and reductase.},
year = {2014}
}
TY - JOUR T1 - Lipid-Modulating Effects of Aqueous Extract of Rubus Occidentalis in Hepatocarcinoma HepG2 Cells AU - Young-Sook Moon AU - Ju-Hee Han AU - Tae-Bum Lee AU - Ji-Wung Kwon AU - Cheol-Hee Choi Y1 - 2014/04/10 PY - 2014 N1 - https://doi.org/10.11648/j.ajbio.20140202.18 DO - 10.11648/j.ajbio.20140202.18 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 64 EP - 69 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20140202.18 AB - Little knowledge exists on the lipid-modulating effect of Rubus occidentalis (RO). The present study investigated the molecular mechanisms of lipid-modulating effects of aqueous extract of RO (ROW) in hepatocarcinoma HepG2 cells. ROW decreased apolipoprotein B100 (ApoB100)/apolipoprotein A-1 (ApoA-1) ratio. ROW increased the expression of LDL-receptor (LDL-R). ROW decreased the gene expression of sterol regulatory element-binding protein 1c (SREBP-1c) as well as fatty acid synthase in a concentration-dependent manner. ROW not only down-regulated gene expression of SREBP-2 and HMG-CoA synthase mRNA expression, but also inhibited HMG-CoA reductase activity in a concentration-dependent manner (IC50 = 240.6 μg/ml). These results indicate that ROW decreases the level of bad cholesterol and increases good cholesterol levels by lowering the gene expression of transcription factors, SREBP-1c and SREBP-2, and by inhibiting the expression and/or activity of their downstream enzymes such as fatty acid synthase and HMG-CoA synthase and reductase. VL - 2 IS - 2 ER -
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