White spot syndrome virus (WSSV) is the causative agent of a disease which has recently caused high shrimp morbidity, mortality and severe damage to shrimp cultures. In this study a strain of WSSV from tiger shrimp Penaeus monodon was used to detect DNA damage in muscle cells by using Comet Assay. The term "comet" refers to the pattern of DNA migration through the electrophoresis gel, which often resembles a comet. The present results declare that Comet Assay is a rapid, simple, visual and sensitive technique for detecting and analyzing DNA in damaged cells. In WSSV infected shrimps, damaged cells looked like a tiny comet under a microscope. When DNA is broken in infected cells it forms a tail that moves away from the unbroken DNA. The amount of DNA damage in cells was estimated from comet tail length as the extent of migration of the genetic material. Significant increases in intensity percentage of DNA tail portion, tail length and tail moments of infected shrimps were observed in comparison with normal healthy shrimps. Furthermore, the clinical signs of white spot disease were also discussed in this study.
| Published in | Animal and Veterinary Sciences (Volume 2, Issue 4) |
| DOI | 10.11648/j.avs.20140204.11 |
| Page(s) | 87-93 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
White Spot Virus, Penaeus Monodon, Clinical Sign, DNA Damage, Comet Assay
| [1] | Chou, H.-Y., C.-Y. Huang, C.-H. Wang, H.-C. Chiang, and C.-F. Lo: Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Dis. Aquat. Org. 23, 165–173, 1995. |
| [2] | Inouye, K., S. Miwa, N. Oseko, H. Nakano, T. Kimura, K. Momoyama, and M. Hiraoka: Mass mortality of cultured kuruma-hrimp Penaeus japonicus in Japan in 1993: electrons microscopic evidence of the causative virus. Fish Pathol. 29, 149–158, 1994 |
| [3] | Flegel, T. W., Special topic review: major viral diseases of the black tiger prawn (Penaeus monodon) in Thailand. World J. Microbiol. Biotechnol. 13, 433–442,1997. |
| [4] | Mai, W. J., and W. N. Wang: Protection of blue shrimp(Litopenaeus stylirostris) against the White Spot Syndrome Virus (WSSV) when injected with shrimp lysozyme. Fish Shellfish Immunol. 28, 727–733, 2010 |
| [5] | Lightner, D. V.: Virus diseases of farmed shrimp in the Western Hem,isphere 1 (the Americas): a review. J. Invertebr. Pathol. 106, 110–130, 2011. |
| [6] | Wongteerasupaya, C., J. E. Vickers, S. Sriurairatana, G. L. Nash, A. Akarajamorn, V. Boonsaeng, S. Panyim, A. Tassanakajon, B. Withyachumnarnkul, and T. W. Flegel: A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon. Dis. Aquat. Org. 21, 69–77, 1995. |
| [7] | Venegas C.A., Nonaka L., Mushiake K., Nishizawa T. & Muroga K. , Quasi-immune response of Penaeus japonicus to penaeid rod-shaped DNA virus (PRDV). Diseases of Aquatic Organisms 42, 83–89, 2000. |
| [8] | Escobedo-Bonilla, V Alday-Sanz3, M Wille1, P Sorgeloos1, M B Pensaert and H J morphogenesis and pathogenesis of white spot syndrome virus. Journal of Fish Diseases 2008, 31, characterization, morphogenesis and pathogenesis of white spot syndrome virus. Journal Nauwynck: A review on the morphology, molecular of Fish Diseases, 31, 1–18, 2008. |
| [9] | Oidtmann, B. and Stentiford, G. D., White Spot Syndrome Virus (WSSV) Concentrations in Crustacean Tissues – A Review of Data Relevant to Assess the Risk Associated with Commodity Trade. Transboundary and Emerging Diseases, 2011. |
| [10] | Lightner, DV., A handbook of pathology and diagnostic procedures for diseases of penaeid shrimp. Section 3 11. World Aquaculture Society, Baton Rouge, LA, 1996. |
| [11] | Revilille, C., AL-Beik Dawn , J., Mrola, M., ,Michele, G. , RAND, W , and Alcivar, A. White spot syndrome virus in frozen shrimp sold at Massachusetts supermarket, J. Shellfish Res. 24(1):285-290. 2005. |
| [12] | Takahashi, Y., T. Itami, M. Kondom, M. Maeda, R. Fuji, S. Tomonaga, K. Supamattaya, and S. Boonyaratpalin, Electron microscopic evidence of baciliform virus infection in Kuruma shrimp (Penaeus japonicus). Fish Pathol. 29, 121–125, 1994. |
| [13] | Nakano, H., H. Koube, S. Umezawa, K. Momoyama, M. Hiraoka, K. Inouye, and N. Oseko: Mass mortalities of cultured kuruma shrimp, Penaeus japonicus, in Japan in: epizootiological survey and infection trails. Fish Pathol. 29, 135–139, 1993. |
| [14] | Karunasagar I., Otta S.K. & Karunasagar I. Histopathological and bacteriological study of white spot syndrome of Penaeus monodon along the west coast of India. Aquaculture, 153, 9–13, 1997. |
| [15] | Sahul-Hameed A.S., Yoganandhan K., Sathish S., Rasheed M., Murugan V. & Jayaraman K. ., White spot syndrome virus (WSSV) in two species of freshwater crabs (Paratelphusa hydrodomus and P. pulvinata). Aquaculture 201, 179–186,2001. |
| [16] | Wang Q., Poulos B.T. & Lightner D.V. , Protein analysis of geographic isolates of shrimp white spot syndrome virus. Archives of Viro. 145, 263–274,2000. |
| [17] | Yoganandhan, K.., Thirupathi, S.,. Biochemical, A.S. Sahul Hameedphysiological and hematological changes in white spot syndrome virus-infected shrimp, Penaeus indicus. Aquacul-ture,221(1-4)1:11,2003. |
| [18] | Kanchanaphum P, Wongteerasupaya C, Sitidilokratana N. Boonsaeng V, Panyim S, Tassanakajon A, Withyachumnarnkul B, Flegel TW Experimental transmission of White Spot Syndrome virus (WSSV) from crabs to shrimp Penaeus monodon. Dis Aquat Org 34:l-7, 1989. |
| [19] | Singh, N.P. , M.T. Mc Coy, R. R. , Tice, E.L., Schnedier, Asimple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175:184-191, 1988. |
| [20] | Lee, R.F., Steinert, S. Use of the single cell gel electrophoresis / Comet Assay for detecting DNA damage in aquatic (marine and freshwater) animals. Mutation Res. 544, 43-64,2003. |
| [21] | Frenzilli, G., Nigro, M., Lyons, B.P. The Comet assay for the evaluation of genotoxic impact in aquatic envronments. Mutation Re. 681, 80-92, 2009 |
| [22] | Haldar S, Maharajan A, Chatterjee S, Hunter SA, Chowdhury N, Identification of Vibrio harveyi as a causative bacterium for a tail rot disease of sea bream Sparus aurata from research hatchery in Malta. Microbiol Res 165: 639-648, 2010. [Pubmed: 20129765]. |
| [23] | Chowdhury N, Asakura M, Neogi SB, Hinenoya A, Haldar S, Development of simple and rapid PCR- fingerprinting methods for Vibrio cholerae on the basis of genetic diversity of the superintegron. J Appl Microbiol. 109: 304-312, 2010. [Pubmed: 20070445]. |
| [24] | Wang, Q., B. L. White, R. M. Redman, and D. L. Lightner. Per os challenge of Litopenaeus vannamei postlarvae and Farfantepenaeus duorarum juveniles with six geographic isolates of white spot syndrome virus. Aquaculture 170:179–194, 1999. |
| [25] | Hossain, M. S., A. Chakraborty, B. Joseph, S. K. Otta, and I. Karunasagar, Detection of new hosts for white spot syndrome virus of shrimp using nested polymerase chain reaction. Aquaculture198:1–11, 2000. |
| [26] | Flegel T.W. & Fegan D.F. , Marine shipping could ballast water and hull fouling be a fomite for disease transmission? In: Proceedings of the Conference on World Aquaculture (ed. by World Aquaculture Society), p. 226. Beijing, China, 2002. |
| [27] | Durand S.V., Tang K.F.J. & Lightner D.V., Frozen commodity shrimp: potential avenue for introduc-tion of white spot syndrome virus and yellow head virus. J. Aquatic Animal Health 12, 128–135,2000 |
| [28] | Lo C.F., Ho C.H., Peng S.E., Chen C.H., Hsu H.C., Chiu Y.L., Chang C.F., Liu K.F., Su M.S., Wang C.H. & Kou G.H., White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods. Dis-eases of Aquatic Organisms 27, 215–225,1996b. |
| [29] | Kasornchandra J., Boonyaratpalin S. & Itami T. Detec-tion of white spot syndrome in cultured penaeid shrimp in Asia: microscopic observation and polymerase chain reaction. Aquaculture 164, 243–251,1998. |
| [30] | Wu J.L., Namikoshi A., Nishizawa T., Mushiake K., Teruya K. & Muroga K, Effects of shrimp density on transmission of penaeid acute viremia in Penaeus japonicus by cannibal-ism and the waterborne route. Diseases of Aquatic Organisms, 47, 129–135,2000. |
| [31] | Wang Y.G., Hassan M.D., Shariff M., Zamri S.M. & Chen X. syndrome virus (WSSV) in cultured Penaeus monodon from peninsular Malaysia with emphasis on pathogenesis and the mechanism of white spot formation. Diseases of Aquatic Or-ganisms 39, 1–11,1999a. |
| [32] | Lightner D.V., Hasson K.W., White B.L. & Redman R.M. , Experimental infection of western hemisphere penaeid shrimp with Asian white spot syndrome virus and Asian yellow head virus. Journal of Aquatic Animal Health 10, 271–281,1998 |
| [33] | Nadala E.C.B. & Loh P.C. (1998) A comparative study of three different isolates of white spot virus. Diseases of Aquatic Organisms 33, 231–234,1998 |
| [34] | Lo C.F., Ho C.H., Chen C.H., Liu K.F., Chiu Y.L., Yeh P.Y., Peng S.E., Hsu H.C., Liu H.C., Chang C.F., Su M.S., Wang C.H. & Kou G.H. , Detection and tissue tropism of white spot syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs. Diseases of Aquatic Organisms 30, 53–72, 1997. |
| [35] | Corsin1, F. , Turnbull, J. F., Hao, N. V., Mohan, C. V., Phi, T. T. , Phuoc, L. H. Tinh, N. T. N. , Morgan, K. L., Risk factors associated with white spot syndrome virus infection in a Vietnamese rice-shrimp farming system, Dis Aquat Org., Vol. 47: 1–12, 2001. |
| [36] | Otta S.K., Shubha G., Joseph B., Chakraborty A., Karunasagar I. , Polymerase chain reaction (PCR) detection of white spot syndrome virus (WSSV) in cultured and wild crustaceans in India. Diseases of Aquatic Organisms38, 67–70,1999. |
| [37] | Sahul-Hameed A.S., Anilkumar M., Raj M.L.S. & Jayaraman K., Studies on the pathogenicity of systemic ectodermal and mesodermal baculovirus and its detection in shrimp by immunological methods. Aquaculture 160, 31–45,1998 |
| [38] | Kiatpathomchai W., Boonsaeng V., Tassanakajon A., Wongteerasupaya C., Jitrapakdee S. & Panyim S. A non-stop, single-tube, semi-nested PCR technique for grading the severity of white spot syndrome virus infections in Penaeus monodon. Diseases of Aquatic Organisms 47, 235–239,2001. |
| [39] | Chou H.Y., Huang C.Y., Wang C.H., Chiang H.C. & Lo C.F. Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Diseases of Aquatic Organisms 23, 165–173,1995. |
| [40] | Flegel T.W. (1997) Special topic review: major viral diseases of the black tiger prawn (Penaeus monodon) in Thailand. World. J. of Microb. Biotechn. 13, 433–442,1997 |
| [41] | Wongteerasupaya C., Vickers J.E., Sriurairatana S., Nash G.L., Akarajamorn A., Boonsaeng V., Panyim S., Tassanakajon A., Withyachumnarnkul B. & Flegel T.W. , A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon. Diseases of Aquatic Organisms, 21, 69–77,1995. |
| [42] | Durand S., Lightner D.V., Redman R.M. & Bonami J.R., Ultrastructure and morphogenesis of white spot syndrome baculovirus. Diseases of Aquatic Organisms,29, 205–211,1997. |
| [43] | Chang P.S., Lo C.F., Wang Y.C. & Kou G.H. Identification of white spot syndrome virus associated baculovirus(WSBV) target organs in the shrimp Penaeus monodon by in situ hybridization. Diseases of Aquatic Organisms 27, 131–139,1996. |
| [44] | Chang P.S., Chen H.C. & Wang Y.C.: Detection of white spot syndrome associated baculovirus in experimentally infected wild shrimp, crabs and lobsters by in situ hybri-dization Aquaculture 164, 233–242, 1998. |
| [45] | Kou G.H., Peng S.E., Chiu Y.L. & Lo C.F. (1998) Tissue distribu-tion of white spot syndrome virus (WSSV) in shrimp and crabs. In: Advances in Shrimp Biotechnology (ed. by T.W. Flegel), pp. 267–271. National Center for Genetic Engineering and Biotechnology, Bangkok, 1998. |
| [46] | Rajendran K.V., Vijayan K.K., Santiago T.C. and Krol R.M., Experimental host range and histopa-thology of white spot syndrome virus (WSSV) infection in shrimp, prawns, crayfish and lobsters from India. J. Fish Diseases 22, 183–191, 1999. |
| [47] | Hassan, G.M. and Mazhar, KH.M, Genotoxicity and Histopathological studies on the liver and kidney of male rats fed on diet containing waste fat released from chicken during grilling progress. Egy. J. Genet. Cytol., 39:205-220,2010 |
| [48] | Olive, P.L.; Banath, J.P. and Durand, R.E. , Detection of etoposide resistance by measuring DNA damage in individual Chinese hamster cells. J. Nat. Cancer Inst., 82: 779-783, 1990. |
| [49] | Lee, R.F. and Steinert, S., Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals. Reviews in Mutat. Res. Elsevier Sci., 544: 43-64, 2003 |
| [50] | Richard F Lee, Steinert, S., Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals. Mutation Res.,544, 43-64,2003. |
| [51] | Yoganandhan, K., Thirupathi, S., Sahul Hameed, A.S. Sahul Hameed, A.S., Biochemical, physiological and hematological changes in white spot syndrome virus-infected shrimp, Penaeus indicus. Aquaculture,221(1-4)1:11,2003. |
APA Style
Hala Ali Abdel-Salam. (2014). Detection of DNA Damage in White Spot Syndrome Virus –Infected Shrimp (Peneaus Mondon) by Using Comet Assay. Animal and Veterinary Sciences, 2(4), 87-93. https://doi.org/10.11648/j.avs.20140204.11
ACS Style
Hala Ali Abdel-Salam. Detection of DNA Damage in White Spot Syndrome Virus –Infected Shrimp (Peneaus Mondon) by Using Comet Assay. Anim. Vet. Sci. 2014, 2(4), 87-93. doi: 10.11648/j.avs.20140204.11
AMA Style
Hala Ali Abdel-Salam. Detection of DNA Damage in White Spot Syndrome Virus –Infected Shrimp (Peneaus Mondon) by Using Comet Assay. Anim Vet Sci. 2014;2(4):87-93. doi: 10.11648/j.avs.20140204.11
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Download@article{10.11648/j.avs.20140204.11,
author = {Hala Ali Abdel-Salam},
title = {Detection of DNA Damage in White Spot Syndrome Virus –Infected Shrimp (Peneaus Mondon) by Using Comet Assay},
journal = {Animal and Veterinary Sciences},
volume = {2},
number = {4},
pages = {87-93},
doi = {10.11648/j.avs.20140204.11},
url = {https://doi.org/10.11648/j.avs.20140204.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140204.11},
abstract = {White spot syndrome virus (WSSV) is the causative agent of a disease which has recently caused high shrimp morbidity, mortality and severe damage to shrimp cultures. In this study a strain of WSSV from tiger shrimp Penaeus monodon was used to detect DNA damage in muscle cells by using Comet Assay. The term "comet" refers to the pattern of DNA migration through the electrophoresis gel, which often resembles a comet. The present results declare that Comet Assay is a rapid, simple, visual and sensitive technique for detecting and analyzing DNA in damaged cells. In WSSV infected shrimps, damaged cells looked like a tiny comet under a microscope. When DNA is broken in infected cells it forms a tail that moves away from the unbroken DNA. The amount of DNA damage in cells was estimated from comet tail length as the extent of migration of the genetic material. Significant increases in intensity percentage of DNA tail portion, tail length and tail moments of infected shrimps were observed in comparison with normal healthy shrimps. Furthermore, the clinical signs of white spot disease were also discussed in this study.},
year = {2014}
}
TY - JOUR T1 - Detection of DNA Damage in White Spot Syndrome Virus –Infected Shrimp (Peneaus Mondon) by Using Comet Assay AU - Hala Ali Abdel-Salam Y1 - 2014/06/10 PY - 2014 N1 - https://doi.org/10.11648/j.avs.20140204.11 DO - 10.11648/j.avs.20140204.11 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 87 EP - 93 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/j.avs.20140204.11 AB - White spot syndrome virus (WSSV) is the causative agent of a disease which has recently caused high shrimp morbidity, mortality and severe damage to shrimp cultures. In this study a strain of WSSV from tiger shrimp Penaeus monodon was used to detect DNA damage in muscle cells by using Comet Assay. The term "comet" refers to the pattern of DNA migration through the electrophoresis gel, which often resembles a comet. The present results declare that Comet Assay is a rapid, simple, visual and sensitive technique for detecting and analyzing DNA in damaged cells. In WSSV infected shrimps, damaged cells looked like a tiny comet under a microscope. When DNA is broken in infected cells it forms a tail that moves away from the unbroken DNA. The amount of DNA damage in cells was estimated from comet tail length as the extent of migration of the genetic material. Significant increases in intensity percentage of DNA tail portion, tail length and tail moments of infected shrimps were observed in comparison with normal healthy shrimps. Furthermore, the clinical signs of white spot disease were also discussed in this study. VL - 2 IS - 4 ER -
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