Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro

Xuejun Zhan; Daze Xie; Xiangrong Shu; You Tang; Nanjin Zhou; Ge Dai

doi:doi:10.11648/j.ejpm.20160402.14

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Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro

Xuejun Zhan, Daze Xie, Xiangrong Shu, You Tang, Nanjin Zhou, Ge Dai

Published in European Journal of Preventive Medicine (Volume 4, Issue 2)

Received: 14 February 2016 Accepted: 10 March 2016 Published: 24 March 2016

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Abstract

This study to investigate predominant anaerobic bacteria and their sensitivity spectrum to antibiotics in female patients with genital tract infections from the local and thus to provide scientific basis for the appropriate use of antibiotics. Anaerobic culture method was used to culture and isolate anaerobes in female patients with genital tract infections. Anaerobes were identified by using micro-biochemistry reacting technique and antibiotic-disc susceptibility test. Antibacterial activity of antibiotics to anaerobes was analyzed by means of Kirby-Bauer testing and broth micro-dilution method. The experiment results showed that 72 strains of obligate anaerobes were isolated from 103 samples, positive rate of 69.90%. The predominant anaerobic bacterial were Gram-negative non-spore bacteroides (28 strains, 38.89%) and anaerobic peptostreptococcus (18 strains, 25%). Sensitivity rate of anaerobic cocci, bacteroides and veillonella to metronidazole was 90.91%, 85.71% and 80% respectively. The drug resistance rates of the three isolated anaerobes were all higher than 60% to amikacin, erythromycin and clindamycin. Comparing the MIC₅₀ and MIC₉₀ values of metronidazole (MTZ), penicillin G (PCG) and lincomycin (LCM) on the isolated anaerobes, MTZ had the lowest MIC₅₀ and MIC₉₀ value. The study suggested that anaerobic infection has become a major pathogenic bacterium of infectious diseases in obstetrics and gynecology. Although MTZ has shown strong antibacterial activity in vitro, a few drug resistant strains appeared in the clinically isolated anaerobes and there is an upward trend of the MIC values of MTZ at different rates.

Published in	European Journal of Preventive Medicine (Volume 4, Issue 2)
DOI	10.11648/j.ejpm.20160402.14
Page(s)	44-49
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Antibiotics, Anaerobic Infection, Female Genital Tract, Antibacterial Activity

References

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APA Style

Xuejun Zhan, Daze Xie, Xiangrong Shu, You Tang, Nanjin Zhou, et al. (2016). Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro. European Journal of Preventive Medicine, 4(2), 44-49. https://doi.org/10.11648/j.ejpm.20160402.14

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ACS Style

Xuejun Zhan; Daze Xie; Xiangrong Shu; You Tang; Nanjin Zhou, et al. Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro. Eur. J. Prev. Med. 2016, 4(2), 44-49. doi: 10.11648/j.ejpm.20160402.14

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AMA Style

Xuejun Zhan, Daze Xie, Xiangrong Shu, You Tang, Nanjin Zhou, et al. Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro. Eur J Prev Med. 2016;4(2):44-49. doi: 10.11648/j.ejpm.20160402.14

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@article{10.11648/j.ejpm.20160402.14,
  author = {Xuejun Zhan and Daze Xie and Xiangrong Shu and You Tang and Nanjin Zhou and Ge Dai},
  title = {Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro},
  journal = {European Journal of Preventive Medicine},
  volume = {4},
  number = {2},
  pages = {44-49},
  doi = {10.11648/j.ejpm.20160402.14},
  url = {https://doi.org/10.11648/j.ejpm.20160402.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejpm.20160402.14},
  abstract = {This study to investigate predominant anaerobic bacteria and their sensitivity spectrum to antibiotics in female patients with genital tract infections from the local and thus to provide scientific basis for the appropriate use of antibiotics. Anaerobic culture method was used to culture and isolate anaerobes in female patients with genital tract infections. Anaerobes were identified by using micro-biochemistry reacting technique and antibiotic-disc susceptibility test. Antibacterial activity of antibiotics to anaerobes was analyzed by means of Kirby-Bauer testing and broth micro-dilution method. The experiment results showed that 72 strains of obligate anaerobes were isolated from 103 samples, positive rate of 69.90%. The predominant anaerobic bacterial were Gram-negative non-spore bacteroides (28 strains, 38.89%) and anaerobic peptostreptococcus (18 strains, 25%). Sensitivity rate of anaerobic cocci, bacteroides and veillonella to metronidazole was 90.91%, 85.71% and 80% respectively. The drug resistance rates of the three isolated anaerobes were all higher than 60% to amikacin, erythromycin and clindamycin. Comparing the MIC50 and MIC90 values of metronidazole (MTZ), penicillin G (PCG) and lincomycin (LCM) on the isolated anaerobes, MTZ had the lowest MIC50 and MIC90 value. The study suggested that anaerobic infection has become a major pathogenic bacterium of infectious diseases in obstetrics and gynecology. Although MTZ has shown strong antibacterial activity in vitro, a few drug resistant strains appeared in the clinically isolated anaerobes and there is an upward trend of the MIC values of MTZ at different rates.},
 year = {2016}
}

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TY  - JOUR
T1  - Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro
AU  - Xuejun Zhan
AU  - Daze Xie
AU  - Xiangrong Shu
AU  - You Tang
AU  - Nanjin Zhou
AU  - Ge Dai
Y1  - 2016/03/24
PY  - 2016
N1  - https://doi.org/10.11648/j.ejpm.20160402.14
DO  - 10.11648/j.ejpm.20160402.14
T2  - European Journal of Preventive Medicine
JF  - European Journal of Preventive Medicine
JO  - European Journal of Preventive Medicine
SP  - 44
EP  - 49
PB  - Science Publishing Group
SN  - 2330-8230
UR  - https://doi.org/10.11648/j.ejpm.20160402.14
AB  - This study to investigate predominant anaerobic bacteria and their sensitivity spectrum to antibiotics in female patients with genital tract infections from the local and thus to provide scientific basis for the appropriate use of antibiotics. Anaerobic culture method was used to culture and isolate anaerobes in female patients with genital tract infections. Anaerobes were identified by using micro-biochemistry reacting technique and antibiotic-disc susceptibility test. Antibacterial activity of antibiotics to anaerobes was analyzed by means of Kirby-Bauer testing and broth micro-dilution method. The experiment results showed that 72 strains of obligate anaerobes were isolated from 103 samples, positive rate of 69.90%. The predominant anaerobic bacterial were Gram-negative non-spore bacteroides (28 strains, 38.89%) and anaerobic peptostreptococcus (18 strains, 25%). Sensitivity rate of anaerobic cocci, bacteroides and veillonella to metronidazole was 90.91%, 85.71% and 80% respectively. The drug resistance rates of the three isolated anaerobes were all higher than 60% to amikacin, erythromycin and clindamycin. Comparing the MIC50 and MIC90 values of metronidazole (MTZ), penicillin G (PCG) and lincomycin (LCM) on the isolated anaerobes, MTZ had the lowest MIC50 and MIC90 value. The study suggested that anaerobic infection has become a major pathogenic bacterium of infectious diseases in obstetrics and gynecology. Although MTZ has shown strong antibacterial activity in vitro, a few drug resistant strains appeared in the clinically isolated anaerobes and there is an upward trend of the MIC values of MTZ at different rates.
VL  - 4
IS  - 2
ER  -

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Author Information

Xuejun Zhan

Key Laboratory of Jiangxi Medical Immunity and Biotherapy, Jiangxi Academy of Medical Science, Nanchang, China
Daze Xie

Key Laboratory of Jiangxi Medical Immunity and Biotherapy, Jiangxi Academy of Medical Science, Nanchang, China
Xiangrong Shu

Department of Cinical Laboratory, Jiangxi Maternal and Children’s Health Care Hospital, Nanchang, China
You Tang

Department of Clinical Laboratory, Pingxiang Hospital of Nanchang University, Pingxiang, China
Nanjin Zhou

Key Laboratory of Jiangxi Medical Immunity and Biotherapy, Jiangxi Academy of Medical Science, Nanchang, China
Ge Dai

Key Laboratory of Jiangxi Medical Immunity and Biotherapy, Jiangxi Academy of Medical Science, Nanchang, China

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Xuejun Zhan, Daze Xie, Xiangrong Shu, You Tang, Nanjin Zhou, et al. (2016). Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro. European Journal of Preventive Medicine, 4(2), 44-49. https://doi.org/10.11648/j.ejpm.20160402.14

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ACS Style

Xuejun Zhan; Daze Xie; Xiangrong Shu; You Tang; Nanjin Zhou, et al. Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro. Eur. J. Prev. Med. 2016, 4(2), 44-49. doi: 10.11648/j.ejpm.20160402.14

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AMA Style

Xuejun Zhan, Daze Xie, Xiangrong Shu, You Tang, Nanjin Zhou, et al. Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro. Eur J Prev Med. 2016;4(2):44-49. doi: 10.11648/j.ejpm.20160402.14

Copy | Download

@article{10.11648/j.ejpm.20160402.14,
  author = {Xuejun Zhan and Daze Xie and Xiangrong Shu and You Tang and Nanjin Zhou and Ge Dai},
  title = {Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro},
  journal = {European Journal of Preventive Medicine},
  volume = {4},
  number = {2},
  pages = {44-49},
  doi = {10.11648/j.ejpm.20160402.14},
  url = {https://doi.org/10.11648/j.ejpm.20160402.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejpm.20160402.14},
  abstract = {This study to investigate predominant anaerobic bacteria and their sensitivity spectrum to antibiotics in female patients with genital tract infections from the local and thus to provide scientific basis for the appropriate use of antibiotics. Anaerobic culture method was used to culture and isolate anaerobes in female patients with genital tract infections. Anaerobes were identified by using micro-biochemistry reacting technique and antibiotic-disc susceptibility test. Antibacterial activity of antibiotics to anaerobes was analyzed by means of Kirby-Bauer testing and broth micro-dilution method. The experiment results showed that 72 strains of obligate anaerobes were isolated from 103 samples, positive rate of 69.90%. The predominant anaerobic bacterial were Gram-negative non-spore bacteroides (28 strains, 38.89%) and anaerobic peptostreptococcus (18 strains, 25%). Sensitivity rate of anaerobic cocci, bacteroides and veillonella to metronidazole was 90.91%, 85.71% and 80% respectively. The drug resistance rates of the three isolated anaerobes were all higher than 60% to amikacin, erythromycin and clindamycin. Comparing the MIC50 and MIC90 values of metronidazole (MTZ), penicillin G (PCG) and lincomycin (LCM) on the isolated anaerobes, MTZ had the lowest MIC50 and MIC90 value. The study suggested that anaerobic infection has become a major pathogenic bacterium of infectious diseases in obstetrics and gynecology. Although MTZ has shown strong antibacterial activity in vitro, a few drug resistant strains appeared in the clinically isolated anaerobes and there is an upward trend of the MIC values of MTZ at different rates.},
 year = {2016}
}

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TY  - JOUR
T1  - Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro
AU  - Xuejun Zhan
AU  - Daze Xie
AU  - Xiangrong Shu
AU  - You Tang
AU  - Nanjin Zhou
AU  - Ge Dai
Y1  - 2016/03/24
PY  - 2016
N1  - https://doi.org/10.11648/j.ejpm.20160402.14
DO  - 10.11648/j.ejpm.20160402.14
T2  - European Journal of Preventive Medicine
JF  - European Journal of Preventive Medicine
JO  - European Journal of Preventive Medicine
SP  - 44
EP  - 49
PB  - Science Publishing Group
SN  - 2330-8230
UR  - https://doi.org/10.11648/j.ejpm.20160402.14
AB  - This study to investigate predominant anaerobic bacteria and their sensitivity spectrum to antibiotics in female patients with genital tract infections from the local and thus to provide scientific basis for the appropriate use of antibiotics. Anaerobic culture method was used to culture and isolate anaerobes in female patients with genital tract infections. Anaerobes were identified by using micro-biochemistry reacting technique and antibiotic-disc susceptibility test. Antibacterial activity of antibiotics to anaerobes was analyzed by means of Kirby-Bauer testing and broth micro-dilution method. The experiment results showed that 72 strains of obligate anaerobes were isolated from 103 samples, positive rate of 69.90%. The predominant anaerobic bacterial were Gram-negative non-spore bacteroides (28 strains, 38.89%) and anaerobic peptostreptococcus (18 strains, 25%). Sensitivity rate of anaerobic cocci, bacteroides and veillonella to metronidazole was 90.91%, 85.71% and 80% respectively. The drug resistance rates of the three isolated anaerobes were all higher than 60% to amikacin, erythromycin and clindamycin. Comparing the MIC50 and MIC90 values of metronidazole (MTZ), penicillin G (PCG) and lincomycin (LCM) on the isolated anaerobes, MTZ had the lowest MIC50 and MIC90 value. The study suggested that anaerobic infection has become a major pathogenic bacterium of infectious diseases in obstetrics and gynecology. Although MTZ has shown strong antibacterial activity in vitro, a few drug resistant strains appeared in the clinically isolated anaerobes and there is an upward trend of the MIC values of MTZ at different rates.
VL  - 4
IS  - 2
ER  -

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