Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch

Ryoko Shimada; Junichi Matsumoto; Mikiharu Doi; Kiyoshi Ebihara

doi:doi:10.11648/j.jfns.20190706.11

| Peer-Reviewed

Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch

Ryoko Shimada, Junichi Matsumoto, Mikiharu Doi, Kiyoshi Ebihara

Published in Journal of Food and Nutrition Sciences (Volume 7, Issue 6)

Received: 8 October 2019 Accepted: 25 October 2019 Published: 31 October 2019

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

It was examined whether undigested protein, namely resistant protein, affects glucagon-like peptide-1 (GLP-1) secretion in rats fed high amylose corn starch (HACS). Rats were fed one of three experimental diets for 28 d: casein without HACS, casein with HACS or dashigara with HACS. Dashigara is produced from Katsuobushi (smoke-dried skipjack tuna) treated with microbial protease. The apparent digestibilities of casein and dashigara are 96.0% and 84.5%, respectively. The amount of rat cecal butyric acid and propionic acid were as follows: the dashigara with HACS group > the casein with HACS group > the casein without HACS group. The dashigara with HACS group had significantly greater cecal butyric acid and lower cecal succinic acid than the casein with HACS group. The GLP-1 concentration in portal vein blood increased as the amount of butyric acid and propionic acid in the cecal contents increased. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments showed that the composition of cecal microbiota differed among the three groups. These results suggest that resistant protein contained in dashigara promotes GLP-1 secretion by controlling fermentation efficiency as well as the fermentation profile of HACS through the changes in cecal microbiota in rats fed HACS.

Published in	Journal of Food and Nutrition Sciences (Volume 7, Issue 6)
DOI	10.11648/j.jfns.20190706.11
Page(s)	79-86
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Glucagon-Like Peptide-1, High Amylose Corn Starch, Resistant Protein, Short Chain Fatty Acids, Cecal Microbiota, Cecal Fermentation

References

[1]	Lei Z, Ting LH, Li S, Chen FQ, Ling QL, Ping JW. Effect of dietary resistant starch on prevention and treatment of obesity-related diseases and its possible mechanisms. Biomed Environ Sci 2015; 28: 91-97.
[2]	Duda-Chodak A, Tarko T, Satora P, Sroka P. Interaction of dietary compounds, especially polyphenols, with the intestinel microbeta: a review. Eur J Nutr 2015; 54: 325-341.
[3]	Jha R, Berrocoso JFD. Dietary fiber and protein fermentation in the intestine of swine and their interactive effects on gut health and on the environment: A review. Anim Feed Sci Technol 2016; 212: 18-26.
[4]	Morita T, Kasaoka S, Ohhashi A, Ikai M, Numasaki Y, Kiriyama S. Resistant proteins alter cecal short-chain fatty acid profiles in rats fed high-amylose cornstarch. J Nutr 1998; 128: 1156–64.
[5]	Miller L, Houghton JA. The micro-Kjeldahl determination of the nitrogen content of amino acids and protein. J Biol Chem 1945; 159: 373-383.
[6]	AOAC (Association of Official Analytical Chemists) 1995. Official Methods of Analysis, 16th Edition. AOAC International, Gaitherburg, MD.
[7]	Reeves PG, Nielsen FH, Fahey GC Jr. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformation of the AIN-76A rodent diet. J Nutr 1993; 123: 1939-1951.
[8]	Ebihara K, Shiraishi R, Okuma K. Hydroxypropyl-modified potato starch increases fecal bile acid excretion in rats. J Nutr 1998; 128: 848-854.
[9]	Walter J, Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Loach DM, Munro K, et al. Detection and indentification of gastrointestinal Lactobacillus species by using denatureing gradient gel electrophoresis and species-specific PCR primers. Appl Environ Microbiol 2000; 66: 297-303.
[10]	Shimada R, Yoshimura M, Murakami K, Ebihara K. Plasma voncentrations of GLP-1 and PYY in rats fed dietary fiber depend on the fermentability of dietary fiber and respond to an altered diet. Int J Clin Nutr Diet 2015; 1: 103.
[11]	Tolhurst G, Heffron H, Lam YS, Parker HE, Habib AM, Diakogiannaki E, et al. Short-chain fatty acids stimulate glucagon-like peptide-1 secretion via the G-protein-coupled receptor FFAR2. Diabetes 2012; 61: 364-371.
[12]	Bolognini D, Tobin AB, Milligan G, Moss CE. The pharmacology and function of receptors for short-chain fatty acids. Mol Pharmacol 2016; 89: 388-398.
[13]	Puddu A, Sanguineti R, Montecucco F, Viviani GL. Evidence for the gut microbiota short-chain fatty acids as key pathophysiological molecules improving diabetes. Mediat Inflamm Review article (9 pages), Article ID 162021, Volume 2014 (2014).
[14]	Brown AJ, Goldsworthy SM, Barnes AA, Eilert MM, Tcheang L, Daniels D, et al. The orphan G protein-coupled receptors GPR4 and GPR43 are activated by propionate and other short chain fatty acids. J Biol Chem 2003; 278: 11312-11319.
[15]	Psichas A, Sleeth ML, Murphy KG, Brooks L, Bewick GA, Hanyaloglu AC, et al. The short chain fatty acid propionate stimulates GLP-1 and PYY secretion via free fatty acid receptor 2 in rodent. Int J Obes (Lond) 2015; 39: 424-429.
[16]	Macfarlane GT, Macfarlane S. Factors affecting fermentation reactions in the large bowel. Proc Nutr Soc 1993; 52: 367-373.
[17]	Hoshi S, Sakata T, Mikuni K, Hashimoto H, Kimura S. Galactosylsucrose and xylosylfructoside alter digestive tract size and concentration of cecal organic acids in rats fed diets containing cholesterol and cholic acid. J Nutr 1994; 124: 52-60.
[18]	Chiang JYL, Pathak P, Liu H, Donepudi A, Ferrell J, Boehme S. Intestinal farnesoid X receptor and Takeda G protein couple receptor 5 signaling in metabolic regulateon. Dig Dis 2017; 35: 241-245.
[19]	Fiorucci S, Biagioli M, Zampella A, Distrutti E. Bile acids activated receptors regulate innate immunity. Front Immunol 2018 Aug 13; 9: 1853.
[20]	Shapiro H, Kolodziejczyk AA, Halstuch D, Elinav E. Bile acids in glucose metabolism in health and disease. J Exp Med 2018; 215: 383-396.
[21]	Chen L, McNulty J, Anderson D, Liu Y, Nystrom C, Bullard S, et al. Cholestyramine reverses hyperglycemia and enhances glucose-stimulated glucagon-like peptide 1 release in Zucker diabetic fatty rats. J Pharmacol Exp Ther. 2010; 334: 164–170.
[22]	Harach T, Pols TW, Nomura M, Maida A, Watanabe M, Auwerx J, et al. TGR5 potentiates GLP-1 secretion in response to anionic exchange resins. Sci Rep 2012; 2: 430.
[23]	Zhang, D.; Leung, P. S. Potential roles of GPR120 and its agonists in the management of diabetes. Drug Des Dev Ther 2014; 8: 1013–1027.
[24]	Wang X, Chan CB. n-3 polyunsaturated fatty acids and insulin secretion. 2015; J Endocrinol 244: R97-R106.
[25]	Rossmeisl M, Macek JZ, Kuda O, Jelenik T, Medrikova D, Stankova B, et al. Metabolic effects of n-3 PUFA as phospholipids are superior to triglycerides in mice fed a high-fat diet: possible role of endocannabinoids. Plos One 7 (2012) e38834.
[26]	Nohr MK, Pedersen MH, Gille A, Egerod KL, Engelstoft MS, Husted AS, et al. GPR41/FFAR3 and GPR43/FFAR2 as cosensors for short-chain fatty acids in enteroendocrine cells vs FFAR3 in enteric neurons and FFAR2 in enteric leukocytes. Endcrinology 2013; 154: 3552-3564.

Cite This Article

Plain Text BibTeX RIS

APA Style

Ryoko Shimada, Junichi Matsumoto, Mikiharu Doi, Kiyoshi Ebihara. (2019). Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch. Journal of Food and Nutrition Sciences, 7(6), 79-86. https://doi.org/10.11648/j.jfns.20190706.11

Copy | Download

ACS Style

Ryoko Shimada; Junichi Matsumoto; Mikiharu Doi; Kiyoshi Ebihara. Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch. J. Food Nutr. Sci. 2019, 7(6), 79-86. doi: 10.11648/j.jfns.20190706.11

Copy | Download

AMA Style

Ryoko Shimada, Junichi Matsumoto, Mikiharu Doi, Kiyoshi Ebihara. Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch. J Food Nutr Sci. 2019;7(6):79-86. doi: 10.11648/j.jfns.20190706.11

Copy | Download

@article{10.11648/j.jfns.20190706.11,
  author = {Ryoko Shimada and Junichi Matsumoto and Mikiharu Doi and Kiyoshi Ebihara},
  title = {Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch},
  journal = {Journal of Food and Nutrition Sciences},
  volume = {7},
  number = {6},
  pages = {79-86},
  doi = {10.11648/j.jfns.20190706.11},
  url = {https://doi.org/10.11648/j.jfns.20190706.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20190706.11},
  abstract = {It was examined whether undigested protein, namely resistant protein, affects glucagon-like peptide-1 (GLP-1) secretion in rats fed high amylose corn starch (HACS). Rats were fed one of three experimental diets for 28 d: casein without HACS, casein with HACS or dashigara with HACS. Dashigara is produced from Katsuobushi (smoke-dried skipjack tuna) treated with microbial protease. The apparent digestibilities of casein and dashigara are 96.0% and 84.5%, respectively. The amount of rat cecal butyric acid and propionic acid were as follows: the dashigara with HACS group > the casein with HACS group > the casein without HACS group. The dashigara with HACS group had significantly greater cecal butyric acid and lower cecal succinic acid than the casein with HACS group. The GLP-1 concentration in portal vein blood increased as the amount of butyric acid and propionic acid in the cecal contents increased. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments showed that the composition of cecal microbiota differed among the three groups. These results suggest that resistant protein contained in dashigara promotes GLP-1 secretion by controlling fermentation efficiency as well as the fermentation profile of HACS through the changes in cecal microbiota in rats fed HACS.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch
AU  - Ryoko Shimada
AU  - Junichi Matsumoto
AU  - Mikiharu Doi
AU  - Kiyoshi Ebihara
Y1  - 2019/10/31
PY  - 2019
N1  - https://doi.org/10.11648/j.jfns.20190706.11
DO  - 10.11648/j.jfns.20190706.11
T2  - Journal of Food and Nutrition Sciences
JF  - Journal of Food and Nutrition Sciences
JO  - Journal of Food and Nutrition Sciences
SP  - 79
EP  - 86
PB  - Science Publishing Group
SN  - 2330-7293
UR  - https://doi.org/10.11648/j.jfns.20190706.11
AB  - It was examined whether undigested protein, namely resistant protein, affects glucagon-like peptide-1 (GLP-1) secretion in rats fed high amylose corn starch (HACS). Rats were fed one of three experimental diets for 28 d: casein without HACS, casein with HACS or dashigara with HACS. Dashigara is produced from Katsuobushi (smoke-dried skipjack tuna) treated with microbial protease. The apparent digestibilities of casein and dashigara are 96.0% and 84.5%, respectively. The amount of rat cecal butyric acid and propionic acid were as follows: the dashigara with HACS group > the casein with HACS group > the casein without HACS group. The dashigara with HACS group had significantly greater cecal butyric acid and lower cecal succinic acid than the casein with HACS group. The GLP-1 concentration in portal vein blood increased as the amount of butyric acid and propionic acid in the cecal contents increased. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments showed that the composition of cecal microbiota differed among the three groups. These results suggest that resistant protein contained in dashigara promotes GLP-1 secretion by controlling fermentation efficiency as well as the fermentation profile of HACS through the changes in cecal microbiota in rats fed HACS.
VL  - 7
IS  - 6
ER  -

Copy | Download

Author Information

Ryoko Shimada

School of Human Science and Environment, University of Hyogo, Himeji, Japan
Junichi Matsumoto

Marutomo Co. Ltd., Iyo, Japan
Mikiharu Doi

Marutomo Co. Ltd., Iyo, Japan
Kiyoshi Ebihara

Faculty of Health Sciences, Osaka Aoyama University, Minoh, Japan

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Ryoko Shimada, Junichi Matsumoto, Mikiharu Doi, Kiyoshi Ebihara. (2019). Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch. Journal of Food and Nutrition Sciences, 7(6), 79-86. https://doi.org/10.11648/j.jfns.20190706.11

Copy | Download

ACS Style

Ryoko Shimada; Junichi Matsumoto; Mikiharu Doi; Kiyoshi Ebihara. Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch. J. Food Nutr. Sci. 2019, 7(6), 79-86. doi: 10.11648/j.jfns.20190706.11

Copy | Download

AMA Style

Ryoko Shimada, Junichi Matsumoto, Mikiharu Doi, Kiyoshi Ebihara. Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch. J Food Nutr Sci. 2019;7(6):79-86. doi: 10.11648/j.jfns.20190706.11

Copy | Download

@article{10.11648/j.jfns.20190706.11,
  author = {Ryoko Shimada and Junichi Matsumoto and Mikiharu Doi and Kiyoshi Ebihara},
  title = {Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch},
  journal = {Journal of Food and Nutrition Sciences},
  volume = {7},
  number = {6},
  pages = {79-86},
  doi = {10.11648/j.jfns.20190706.11},
  url = {https://doi.org/10.11648/j.jfns.20190706.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20190706.11},
  abstract = {It was examined whether undigested protein, namely resistant protein, affects glucagon-like peptide-1 (GLP-1) secretion in rats fed high amylose corn starch (HACS). Rats were fed one of three experimental diets for 28 d: casein without HACS, casein with HACS or dashigara with HACS. Dashigara is produced from Katsuobushi (smoke-dried skipjack tuna) treated with microbial protease. The apparent digestibilities of casein and dashigara are 96.0% and 84.5%, respectively. The amount of rat cecal butyric acid and propionic acid were as follows: the dashigara with HACS group > the casein with HACS group > the casein without HACS group. The dashigara with HACS group had significantly greater cecal butyric acid and lower cecal succinic acid than the casein with HACS group. The GLP-1 concentration in portal vein blood increased as the amount of butyric acid and propionic acid in the cecal contents increased. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments showed that the composition of cecal microbiota differed among the three groups. These results suggest that resistant protein contained in dashigara promotes GLP-1 secretion by controlling fermentation efficiency as well as the fermentation profile of HACS through the changes in cecal microbiota in rats fed HACS.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch
AU  - Ryoko Shimada
AU  - Junichi Matsumoto
AU  - Mikiharu Doi
AU  - Kiyoshi Ebihara
Y1  - 2019/10/31
PY  - 2019
N1  - https://doi.org/10.11648/j.jfns.20190706.11
DO  - 10.11648/j.jfns.20190706.11
T2  - Journal of Food and Nutrition Sciences
JF  - Journal of Food and Nutrition Sciences
JO  - Journal of Food and Nutrition Sciences
SP  - 79
EP  - 86
PB  - Science Publishing Group
SN  - 2330-7293
UR  - https://doi.org/10.11648/j.jfns.20190706.11
AB  - It was examined whether undigested protein, namely resistant protein, affects glucagon-like peptide-1 (GLP-1) secretion in rats fed high amylose corn starch (HACS). Rats were fed one of three experimental diets for 28 d: casein without HACS, casein with HACS or dashigara with HACS. Dashigara is produced from Katsuobushi (smoke-dried skipjack tuna) treated with microbial protease. The apparent digestibilities of casein and dashigara are 96.0% and 84.5%, respectively. The amount of rat cecal butyric acid and propionic acid were as follows: the dashigara with HACS group > the casein with HACS group > the casein without HACS group. The dashigara with HACS group had significantly greater cecal butyric acid and lower cecal succinic acid than the casein with HACS group. The GLP-1 concentration in portal vein blood increased as the amount of butyric acid and propionic acid in the cecal contents increased. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments showed that the composition of cecal microbiota differed among the three groups. These results suggest that resistant protein contained in dashigara promotes GLP-1 secretion by controlling fermentation efficiency as well as the fermentation profile of HACS through the changes in cecal microbiota in rats fed HACS.
VL  - 7
IS  - 6
ER  -

Copy | Download