Anthocyanins are colored bioactive phytochemicals in the class of flavonoids with high potential as dietary antioxidants. Demand for anthocyanins in functional food and nutraceutical industries has continued to increase over the past decades. However, sources of anthocyanins for commercial exploitation are currently limited. Tropical Africa and Uganda in particular, harbors many plant species with potential to serve as new sources of anthocyanins. In this study, the anthocyanin profile and antioxidant activity of edible leaves of Dissotis brazzae, a plant of tropical African origin, were investigated. Two known anthocyanins, (1) cyanidin 3-O-β-glucopyranoside (1.28±0.44 mg/g fr. wt.) and (2) cyanidin 3-O-α-rhamnopyranoside (1.89±0.42 mg/g fr. wt.), were isolated from D. brazzae purple leaves by a combination of chromatographic techniques. Their structures were elucidated mainly by homo- and heteronuclear NMR spectroscopy and online HPLC/UV-Vis spectroscopy. The isolated anthocyanins showed appreciable level of antioxidant activity against DPPH, with 1 (IC50=9.2±0.2 μg/ml) showing slightly higher antioxidant potency than 2 (IC50=14.0±0.6 μg/ml). However, quercetin (IC50=4.5±0.4 μg/ml) used as a reference showed a higher antioxidant activity than 1 and 2. The total anthocyanin content of D. brazzae leaves (317 mg/100g) was within the range (80 – 680 mg/100g) of anthocyanins content in most dietary sources. This is the first report of fully characterised anthocyanins from the genus Dissotis, which may be of taxonomic importance. A new dietary source of the rare cyanidin 3-O-α-rhamnopyranoside has been identified.
| Published in | Journal of Food and Nutrition Sciences (Volume 8, Issue 6) |
| DOI | 10.11648/j.jfns.20200806.11 |
| Page(s) | 139-145 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2020. Published by Science Publishing Group |
Dissotis brazzae, Anthocyanins, Nutraceutical, Colorant, Dietary Antioxidant, Functional Food
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APA Style
Christopher Adaku, Irene Skaar, Hannington Gumisiriza, Robert Byamukama, Monica Jordheim, et al. (2020). Anthocyanin Profile and Antioxidant Activity of Edible Leaves of Dissotis brazzae Cogn (Melastomataceae). Journal of Food and Nutrition Sciences, 8(6), 139-145. https://doi.org/10.11648/j.jfns.20200806.11
ACS Style
Christopher Adaku; Irene Skaar; Hannington Gumisiriza; Robert Byamukama; Monica Jordheim, et al. Anthocyanin Profile and Antioxidant Activity of Edible Leaves of Dissotis brazzae Cogn (Melastomataceae). J. Food Nutr. Sci. 2020, 8(6), 139-145. doi: 10.11648/j.jfns.20200806.11
AMA Style
Christopher Adaku, Irene Skaar, Hannington Gumisiriza, Robert Byamukama, Monica Jordheim, et al. Anthocyanin Profile and Antioxidant Activity of Edible Leaves of Dissotis brazzae Cogn (Melastomataceae). J Food Nutr Sci. 2020;8(6):139-145. doi: 10.11648/j.jfns.20200806.11
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Download@article{10.11648/j.jfns.20200806.11,
author = {Christopher Adaku and Irene Skaar and Hannington Gumisiriza and Robert Byamukama and Monica Jordheim and Oyvind Moksheim Andersen},
title = {Anthocyanin Profile and Antioxidant Activity of Edible Leaves of Dissotis brazzae Cogn (Melastomataceae)},
journal = {Journal of Food and Nutrition Sciences},
volume = {8},
number = {6},
pages = {139-145},
doi = {10.11648/j.jfns.20200806.11},
url = {https://doi.org/10.11648/j.jfns.20200806.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20200806.11},
abstract = {Anthocyanins are colored bioactive phytochemicals in the class of flavonoids with high potential as dietary antioxidants. Demand for anthocyanins in functional food and nutraceutical industries has continued to increase over the past decades. However, sources of anthocyanins for commercial exploitation are currently limited. Tropical Africa and Uganda in particular, harbors many plant species with potential to serve as new sources of anthocyanins. In this study, the anthocyanin profile and antioxidant activity of edible leaves of Dissotis brazzae, a plant of tropical African origin, were investigated. Two known anthocyanins, (1) cyanidin 3-O-β-glucopyranoside (1.28±0.44 mg/g fr. wt.) and (2) cyanidin 3-O-α-rhamnopyranoside (1.89±0.42 mg/g fr. wt.), were isolated from D. brazzae purple leaves by a combination of chromatographic techniques. Their structures were elucidated mainly by homo- and heteronuclear NMR spectroscopy and online HPLC/UV-Vis spectroscopy. The isolated anthocyanins showed appreciable level of antioxidant activity against DPPH, with 1 (IC50=9.2±0.2 μg/ml) showing slightly higher antioxidant potency than 2 (IC50=14.0±0.6 μg/ml). However, quercetin (IC50=4.5±0.4 μg/ml) used as a reference showed a higher antioxidant activity than 1 and 2. The total anthocyanin content of D. brazzae leaves (317 mg/100g) was within the range (80 – 680 mg/100g) of anthocyanins content in most dietary sources. This is the first report of fully characterised anthocyanins from the genus Dissotis, which may be of taxonomic importance. A new dietary source of the rare cyanidin 3-O-α-rhamnopyranoside has been identified.},
year = {2020}
}
TY - JOUR T1 - Anthocyanin Profile and Antioxidant Activity of Edible Leaves of Dissotis brazzae Cogn (Melastomataceae) AU - Christopher Adaku AU - Irene Skaar AU - Hannington Gumisiriza AU - Robert Byamukama AU - Monica Jordheim AU - Oyvind Moksheim Andersen Y1 - 2020/11/27 PY - 2020 N1 - https://doi.org/10.11648/j.jfns.20200806.11 DO - 10.11648/j.jfns.20200806.11 T2 - Journal of Food and Nutrition Sciences JF - Journal of Food and Nutrition Sciences JO - Journal of Food and Nutrition Sciences SP - 139 EP - 145 PB - Science Publishing Group SN - 2330-7293 UR - https://doi.org/10.11648/j.jfns.20200806.11 AB - Anthocyanins are colored bioactive phytochemicals in the class of flavonoids with high potential as dietary antioxidants. Demand for anthocyanins in functional food and nutraceutical industries has continued to increase over the past decades. However, sources of anthocyanins for commercial exploitation are currently limited. Tropical Africa and Uganda in particular, harbors many plant species with potential to serve as new sources of anthocyanins. In this study, the anthocyanin profile and antioxidant activity of edible leaves of Dissotis brazzae, a plant of tropical African origin, were investigated. Two known anthocyanins, (1) cyanidin 3-O-β-glucopyranoside (1.28±0.44 mg/g fr. wt.) and (2) cyanidin 3-O-α-rhamnopyranoside (1.89±0.42 mg/g fr. wt.), were isolated from D. brazzae purple leaves by a combination of chromatographic techniques. Their structures were elucidated mainly by homo- and heteronuclear NMR spectroscopy and online HPLC/UV-Vis spectroscopy. The isolated anthocyanins showed appreciable level of antioxidant activity against DPPH, with 1 (IC50=9.2±0.2 μg/ml) showing slightly higher antioxidant potency than 2 (IC50=14.0±0.6 μg/ml). However, quercetin (IC50=4.5±0.4 μg/ml) used as a reference showed a higher antioxidant activity than 1 and 2. The total anthocyanin content of D. brazzae leaves (317 mg/100g) was within the range (80 – 680 mg/100g) of anthocyanins content in most dietary sources. This is the first report of fully characterised anthocyanins from the genus Dissotis, which may be of taxonomic importance. A new dietary source of the rare cyanidin 3-O-α-rhamnopyranoside has been identified. VL - 8 IS - 6 ER -
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