Malling Increasing demands for apple across the world requires its rapid production. Agricultural biotechnology has met this need and has prevented some problems during adolescence period and long generation. We investigated embryonic and non-embryonic callus through cultivating apical and lateral buds on different treatments with MS base culture and BAP (0-2.5 mg/l) and IBA (0-5 mg/l) hormones Kin and NAA hormones. The highest percent of embryogenesis related to T11 treatment (IBA 4 mg/l, BAP 1 mg/l) and T23 (IBA 4 mg/l, BAP 2.5 mg/l). The embryos were in corpuscular stage. Then third sub-culture was performed and all calluses and embryos were delivered to their corresponding treatments. K5 (NAA 1 mg/l, Kin 0.5 mg/l) and K8 (NAA 3 mg/l, Kin 1 mg/l) yielded the highest amount of callus (5.3 mm). The embryos remained in corpuscular stage and they did not show reproductive ability and developmental process. After preparation of artificial seed no regeneration was seen. By embryogenesis of MM.106 and M.26, the purpose of this research is improving a protocol for generation of two somatic cultivars.
| Published in | Journal of Plant Sciences (Volume 4, Issue 3) |
| DOI | 10.11648/j.jps.20160403.13 |
| Page(s) | 46-51 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Malling, Synthetic Seed, Tissue Culture
| [1] | Ammirato, Philip V (1986). "Organizational events during somatic embryogenesis." Plant biology (USA). |
| [2] | Barbotin J, Timbert R (1996). bioencapsulation of carrot somatic embryo. progress in biotechnology.11, 641-648. |
| [3] | Brischia R, Piccioni E. (2002). Micropropagation and synthetic seed in M.26 apple rootstock. Plant cell tissue and organculture.68, 137-141. |
| [4] | Daigny, Paul G, 1996. Factors influencing secondry somatic embrogenesis in Malus x domestica Borkh. plant cell reports. 16, 153-157. |
| [5] | Faisal M, Naseem A (2006). In vitro plant regeneration formal ginate encapsulated microcuttings of Rauvolfiatetraphylla. L. Agric environ sci1-6. |
| [6] | Fasolo, Malavasi F, Predieri S 1989. "Cultivar dependent responses to regeneration from leaves in apple." I International Symposium on In Vitro Culture and Horticultural Breeding 280. |
| [7] | Frey, Les, Yehoshua S, Jules J (1992). "Somatic embryogenesis in carnation." Hort Science 27.1: 63-65. |
| [8] | Fridlender M, Lev-Yadun S, Baburek I, Angelis K, Levy, A (1996). Cell divisions in cotyledons after germination: localization, time course and utilization for a mutagenesis assay. Planta, 199(2), 307-313. |
| [9] | James, David J, Andrew J. Passey, Charles D (1984). "Adventitious embryogenesis and the in vitro culture of apple seed parts." Journal of plant physiology 115.3: 217-229. |
| [10] | Kohlenbach H W (1985). "Fundamental and applied aspects of in vitro plant regeneration by somatic embryogenesis." Advances in agricultural biotechnology. |
| [11] | Krikorian, Abraham D, Steward F. C (1978). "IS GRAVITY A MORPHOLOGICAL DETERMINANT IN PLANTS AT THE CELLULAR LEVEL2." Life Sciences and Space Research: Proceedings of the Open Meeting of the Working Group on Space Biology of the Twenty-First Plenary Meeting of COSPAR, Innsbruck, Austria, 29 May-10 June. Vol. 17. Elsevier, 2013. |
| [12] | Mehra P N, Sachdeva (1984). "Embryogenesis in apple in vitro." Phytomorphology. |
| [13] | Micheli M, Pellegrino S, Piccioni E (2002). Effect of double encapsulation and coating on synthetic seed in M.26apple rootstock. Journal of microencapsulation.19: 347-356. |
| [14] | Mingozzi M, Morini S (2009). "In vitro cultivation of donor quince shoots affects subsequent morphogenesis in leaf explants." Biologiaplantarum 53.1: 141-144. |
| [15] | Murashige, T., skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures, plant physiology. 15, 473-49. |
| [16] | Overvoorde, Paul J, Howard D, Grimes (1994). "The role of calcium and calmodulin in carrot somatic embryogenesis." Plant and cell physiology 35.2: 135-144. |
| [17] | Rady (2004). Synthetic seed technology for encapsulation and regrowth of in vitroderived Gypsophila paniculata L. Arab J. biotech.7, 251-264. |
| [18] | Rai, Manoj K (2009) "The encapsulation technology in fruit plants—a review." Biotechnology advances 27.6: 671-679. |
| [19] | Ravi, Dhabhai, Prakash (2012)."Production and applications of artificial seeds: A review." Int. Res. J. Biol. Sci 1: 74-78. |
| [20] | Room, Peter, Jim Hanan, Przemyslaw Prusinkiewicz (1996). "Virtual plants: new perspectives for ecologists, pathologists and agricultural scientists." Trends in Plant Science 1.1 33-38. |
| [21] | Sarmah D. K, Borthakur M, Borua P K (2010). Artificial seed production from encapsulated PLBs regenerated from leaf base of Vanda coerulea Grifft. ex. Lindl. – an endangered orchid. Current science. 98(5), 686-690. |
| [22] | Sharp WR, Evans,. Sondahl M R (1982). "Application of somatic embryogenesis to crop improvement." Plant tissue culture proceedings, 5th International Congress of Plant Tissue and Cell Culture held at Tokyo and Lake Yamanake, Japan, July 11-16. |
| [23] | Slade, D, Fujii, J A, Redenbaugh K (1989). A method for the encapsulation of somatic embryos. Tissue culture methods.12, 179-183. |
| [24] | Standardi A, Maurizio M (2013). Encapsulation of in vitro –derived explants. Methods in molecular biology.994, (397-418). |
| [25] | Tisserat B, Esan E B, Murashige T (1979). "Somatic embryogenesis in angiosperms." Horticultural Reviews, Volume 1: 1-78. |
| [26] | Tsvetkov I, Hausman J (2005). In vitro regeneration from alginate-encapsulated microcuttings of Quercus sp. Scientia Horticulturae.103, 503-507. |
| [27] | Yao, Jia-Long, et al (1996) "Transformation of citrus embryogenic cells using particle bombardment and production of transgenic embryos." Plant Science 113.2 175-183. |
| [28] | Yepez, Charlotte, Erik C (2007) "Micropropagación de Pothomorpheumbellata (L.) Miq. víaorganogénesisdirecta." Revista Cubana de Plantas Medicinales 12.4: 0-0. |
APA Style
Zarinkamar Elham, Nejad Satari Taher. (2016). Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106. Journal of Plant Sciences, 4(3), 46-51. https://doi.org/10.11648/j.jps.20160403.13
ACS Style
Zarinkamar Elham; Nejad Satari Taher. Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106. J. Plant Sci. 2016, 4(3), 46-51. doi: 10.11648/j.jps.20160403.13
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.jps.20160403.13,
author = {Zarinkamar Elham and Nejad Satari Taher},
title = {Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106},
journal = {Journal of Plant Sciences},
volume = {4},
number = {3},
pages = {46-51},
doi = {10.11648/j.jps.20160403.13},
url = {https://doi.org/10.11648/j.jps.20160403.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20160403.13},
abstract = {Malling Increasing demands for apple across the world requires its rapid production. Agricultural biotechnology has met this need and has prevented some problems during adolescence period and long generation. We investigated embryonic and non-embryonic callus through cultivating apical and lateral buds on different treatments with MS base culture and BAP (0-2.5 mg/l) and IBA (0-5 mg/l) hormones Kin and NAA hormones. The highest percent of embryogenesis related to T11 treatment (IBA 4 mg/l, BAP 1 mg/l) and T23 (IBA 4 mg/l, BAP 2.5 mg/l). The embryos were in corpuscular stage. Then third sub-culture was performed and all calluses and embryos were delivered to their corresponding treatments. K5 (NAA 1 mg/l, Kin 0.5 mg/l) and K8 (NAA 3 mg/l, Kin 1 mg/l) yielded the highest amount of callus (5.3 mm). The embryos remained in corpuscular stage and they did not show reproductive ability and developmental process. After preparation of artificial seed no regeneration was seen. By embryogenesis of MM.106 and M.26, the purpose of this research is improving a protocol for generation of two somatic cultivars.},
year = {2016}
}
TY - JOUR T1 - Optimizing of Synthetic Seed of Malling apple (Malusdomestica) Rootstocks cv. M26 and cv. MM106 AU - Zarinkamar Elham AU - Nejad Satari Taher Y1 - 2016/05/11 PY - 2016 N1 - https://doi.org/10.11648/j.jps.20160403.13 DO - 10.11648/j.jps.20160403.13 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 46 EP - 51 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20160403.13 AB - Malling Increasing demands for apple across the world requires its rapid production. Agricultural biotechnology has met this need and has prevented some problems during adolescence period and long generation. We investigated embryonic and non-embryonic callus through cultivating apical and lateral buds on different treatments with MS base culture and BAP (0-2.5 mg/l) and IBA (0-5 mg/l) hormones Kin and NAA hormones. The highest percent of embryogenesis related to T11 treatment (IBA 4 mg/l, BAP 1 mg/l) and T23 (IBA 4 mg/l, BAP 2.5 mg/l). The embryos were in corpuscular stage. Then third sub-culture was performed and all calluses and embryos were delivered to their corresponding treatments. K5 (NAA 1 mg/l, Kin 0.5 mg/l) and K8 (NAA 3 mg/l, Kin 1 mg/l) yielded the highest amount of callus (5.3 mm). The embryos remained in corpuscular stage and they did not show reproductive ability and developmental process. After preparation of artificial seed no regeneration was seen. By embryogenesis of MM.106 and M.26, the purpose of this research is improving a protocol for generation of two somatic cultivars. VL - 4 IS - 3 ER -
Email: