Atropisomeric Separation of PCB-95 by HPLC

Prabha Ranasinghe; Christopher Olivares; William Champion Jr; Cindy Lee

doi:doi:10.11648/j.sjc.20190702.12

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Atropisomeric Separation of PCB-95 by HPLC

Prabha Ranasinghe, Christopher Olivares, William Champion Jr, Cindy Lee

Published in Science Journal of Chemistry (Volume 7, Issue 2)

Received: 3 February 2019 Accepted: 20 March 2019 Published: 29 June 2019

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Abstract

2,2’,3,5’,6-Pentachlorobiphenyl (PCB-95) is an environmentally significant chiral PCB, of which enantioselective toxicity, biodegradation and chiral stability studies have been limited to date, as no commercially available enantiomers exist for PCB-95 and due to the lack of an efficient preparatory chiral separation method. A selective, sensitive, and rapid high-performance liquid chromatography with UV detection (HPLC-UV) method has been developed and validated for the chromatographic separation and quantitation of PCB-95 enantiomers. In this study, we resolved enantiomers of PCB-95 using a cellulose tris (4-methylbenzoate) Chiralcel OJ- H column. After evaluating mobile phase compositions and temperatures, optimum separation and detection were obtained with isocratic 100% n-hexane as the mobile phase, a column temperature of 20°C, a flow rate of 1 mL/min, and a detection wavelength of 280 nm. The total run time was 8 minutes. Enantiomer purity was confirmed using enantioselective gas capillary chromatography-electron capture detection. The developed method was validated as per International Conference on Harmonization (ICH) guidelines with respect to limit of detection, limit of quantification, precision, linearity, robustness and ruggedness.

Published in	Science Journal of Chemistry (Volume 7, Issue 2)
DOI	10.11648/j.sjc.20190702.12
Page(s)	39-48
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Enantioselective Studies, 2, 2’, 3, 5’, 6-Pentachlorobiphenyl, Chiralcel OJ - H, Liquid Chromatography

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APA Style

Prabha Ranasinghe, Christopher Olivares, William Champion Jr, Cindy Lee. (2019). Atropisomeric Separation of PCB-95 by HPLC. Science Journal of Chemistry, 7(2), 39-48. https://doi.org/10.11648/j.sjc.20190702.12

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ACS Style

Prabha Ranasinghe; Christopher Olivares; William Champion Jr; Cindy Lee. Atropisomeric Separation of PCB-95 by HPLC. Sci. J. Chem. 2019, 7(2), 39-48. doi: 10.11648/j.sjc.20190702.12

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AMA Style

Prabha Ranasinghe, Christopher Olivares, William Champion Jr, Cindy Lee. Atropisomeric Separation of PCB-95 by HPLC. Sci J Chem. 2019;7(2):39-48. doi: 10.11648/j.sjc.20190702.12

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@article{10.11648/j.sjc.20190702.12,
  author = {Prabha Ranasinghe and Christopher Olivares and William Champion Jr and Cindy Lee},
  title = {Atropisomeric Separation of PCB-95 by HPLC},
  journal = {Science Journal of Chemistry},
  volume = {7},
  number = {2},
  pages = {39-48},
  doi = {10.11648/j.sjc.20190702.12},
  url = {https://doi.org/10.11648/j.sjc.20190702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20190702.12},
  abstract = {2,2’,3,5’,6-Pentachlorobiphenyl (PCB-95) is an environmentally significant chiral PCB, of which enantioselective toxicity, biodegradation and chiral stability studies have been limited to date, as no commercially available enantiomers exist for PCB-95 and due to the lack of an efficient preparatory chiral separation method. A selective, sensitive, and rapid high-performance liquid chromatography with UV detection (HPLC-UV) method has been developed and validated for the chromatographic separation and quantitation of PCB-95 enantiomers. In this study, we resolved enantiomers of PCB-95 using a cellulose tris (4-methylbenzoate) Chiralcel OJ- H column. After evaluating mobile phase compositions and temperatures, optimum separation and detection were obtained with isocratic 100% n-hexane as the mobile phase, a column temperature of 20°C, a flow rate of 1 mL/min, and a detection wavelength of 280 nm. The total run time was 8 minutes. Enantiomer purity was confirmed using enantioselective gas capillary chromatography-electron capture detection. The developed method was validated as per International Conference on Harmonization (ICH) guidelines with respect to limit of detection, limit of quantification, precision, linearity, robustness and ruggedness.},
 year = {2019}
}

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TY  - JOUR
T1  - Atropisomeric Separation of PCB-95 by HPLC
AU  - Prabha Ranasinghe
AU  - Christopher Olivares
AU  - William Champion Jr
AU  - Cindy Lee
Y1  - 2019/06/29
PY  - 2019
N1  - https://doi.org/10.11648/j.sjc.20190702.12
DO  - 10.11648/j.sjc.20190702.12
T2  - Science Journal of Chemistry
JF  - Science Journal of Chemistry
JO  - Science Journal of Chemistry
SP  - 39
EP  - 48
PB  - Science Publishing Group
SN  - 2330-099X
UR  - https://doi.org/10.11648/j.sjc.20190702.12
AB  - 2,2’,3,5’,6-Pentachlorobiphenyl (PCB-95) is an environmentally significant chiral PCB, of which enantioselective toxicity, biodegradation and chiral stability studies have been limited to date, as no commercially available enantiomers exist for PCB-95 and due to the lack of an efficient preparatory chiral separation method. A selective, sensitive, and rapid high-performance liquid chromatography with UV detection (HPLC-UV) method has been developed and validated for the chromatographic separation and quantitation of PCB-95 enantiomers. In this study, we resolved enantiomers of PCB-95 using a cellulose tris (4-methylbenzoate) Chiralcel OJ- H column. After evaluating mobile phase compositions and temperatures, optimum separation and detection were obtained with isocratic 100% n-hexane as the mobile phase, a column temperature of 20°C, a flow rate of 1 mL/min, and a detection wavelength of 280 nm. The total run time was 8 minutes. Enantiomer purity was confirmed using enantioselective gas capillary chromatography-electron capture detection. The developed method was validated as per International Conference on Harmonization (ICH) guidelines with respect to limit of detection, limit of quantification, precision, linearity, robustness and ruggedness.
VL  - 7
IS  - 2
ER  -

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Author Information

Prabha Ranasinghe

Environmental Toxicology Program, Clemson University, Clemson, USA
Christopher Olivares

Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, USA
William Champion Jr

Chiral Technologies Inc., West Chester, USA
Cindy Lee

Environmental Toxicology Program, Clemson University, Clemson, USA

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Plain Text BibTeX RIS

APA Style

Prabha Ranasinghe, Christopher Olivares, William Champion Jr, Cindy Lee. (2019). Atropisomeric Separation of PCB-95 by HPLC. Science Journal of Chemistry, 7(2), 39-48. https://doi.org/10.11648/j.sjc.20190702.12

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ACS Style

Prabha Ranasinghe; Christopher Olivares; William Champion Jr; Cindy Lee. Atropisomeric Separation of PCB-95 by HPLC. Sci. J. Chem. 2019, 7(2), 39-48. doi: 10.11648/j.sjc.20190702.12

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AMA Style

Prabha Ranasinghe, Christopher Olivares, William Champion Jr, Cindy Lee. Atropisomeric Separation of PCB-95 by HPLC. Sci J Chem. 2019;7(2):39-48. doi: 10.11648/j.sjc.20190702.12

Copy | Download

@article{10.11648/j.sjc.20190702.12,
  author = {Prabha Ranasinghe and Christopher Olivares and William Champion Jr and Cindy Lee},
  title = {Atropisomeric Separation of PCB-95 by HPLC},
  journal = {Science Journal of Chemistry},
  volume = {7},
  number = {2},
  pages = {39-48},
  doi = {10.11648/j.sjc.20190702.12},
  url = {https://doi.org/10.11648/j.sjc.20190702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20190702.12},
  abstract = {2,2’,3,5’,6-Pentachlorobiphenyl (PCB-95) is an environmentally significant chiral PCB, of which enantioselective toxicity, biodegradation and chiral stability studies have been limited to date, as no commercially available enantiomers exist for PCB-95 and due to the lack of an efficient preparatory chiral separation method. A selective, sensitive, and rapid high-performance liquid chromatography with UV detection (HPLC-UV) method has been developed and validated for the chromatographic separation and quantitation of PCB-95 enantiomers. In this study, we resolved enantiomers of PCB-95 using a cellulose tris (4-methylbenzoate) Chiralcel OJ- H column. After evaluating mobile phase compositions and temperatures, optimum separation and detection were obtained with isocratic 100% n-hexane as the mobile phase, a column temperature of 20°C, a flow rate of 1 mL/min, and a detection wavelength of 280 nm. The total run time was 8 minutes. Enantiomer purity was confirmed using enantioselective gas capillary chromatography-electron capture detection. The developed method was validated as per International Conference on Harmonization (ICH) guidelines with respect to limit of detection, limit of quantification, precision, linearity, robustness and ruggedness.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Atropisomeric Separation of PCB-95 by HPLC
AU  - Prabha Ranasinghe
AU  - Christopher Olivares
AU  - William Champion Jr
AU  - Cindy Lee
Y1  - 2019/06/29
PY  - 2019
N1  - https://doi.org/10.11648/j.sjc.20190702.12
DO  - 10.11648/j.sjc.20190702.12
T2  - Science Journal of Chemistry
JF  - Science Journal of Chemistry
JO  - Science Journal of Chemistry
SP  - 39
EP  - 48
PB  - Science Publishing Group
SN  - 2330-099X
UR  - https://doi.org/10.11648/j.sjc.20190702.12
AB  - 2,2’,3,5’,6-Pentachlorobiphenyl (PCB-95) is an environmentally significant chiral PCB, of which enantioselective toxicity, biodegradation and chiral stability studies have been limited to date, as no commercially available enantiomers exist for PCB-95 and due to the lack of an efficient preparatory chiral separation method. A selective, sensitive, and rapid high-performance liquid chromatography with UV detection (HPLC-UV) method has been developed and validated for the chromatographic separation and quantitation of PCB-95 enantiomers. In this study, we resolved enantiomers of PCB-95 using a cellulose tris (4-methylbenzoate) Chiralcel OJ- H column. After evaluating mobile phase compositions and temperatures, optimum separation and detection were obtained with isocratic 100% n-hexane as the mobile phase, a column temperature of 20°C, a flow rate of 1 mL/min, and a detection wavelength of 280 nm. The total run time was 8 minutes. Enantiomer purity was confirmed using enantioselective gas capillary chromatography-electron capture detection. The developed method was validated as per International Conference on Harmonization (ICH) guidelines with respect to limit of detection, limit of quantification, precision, linearity, robustness and ruggedness.
VL  - 7
IS  - 2
ER  -

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