Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide

Vinayak Ambike; Anant Argekar

doi:doi:10.11648/j.sjee.20170506.14

| Peer-Reviewed

Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide

Vinayak Ambike, Anant Argekar

Published in Science Journal of Energy Engineering (Volume 5, Issue 6)

Received: 30 June 2017 Accepted: 20 July 2017 Published: 1 December 2017

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

A new, fast, accurate, reliable and stability indicating HPLC method for the determination of Diflurobenzuron (DFB), in technical and formulation samples in presence of related and degraded impurities has been developed using intersil – 3 stainless steel C. 18 column (5 µm, 250 mm length x 4.6 mm id), acetonitrite: water: 1.4 dioxane (58:39:0.03 v/v) as mobile phase and diphenyl as internal standard. The flow rate of the mobile phase was 2 cm³ min^-1. Detection was carried out at 260 mm using UV detector. The retention times were 1.55 min, 1.75 min, 6.3 min, 7.9 min and 10.7 min for 2, 6 diflurobenzoic acid, 2, 6 diflurobuzamide, diflurobenzuron, difur (an impurity) and diphenyl (internal standard) respectively. The linearity range of DFB was 0.5 to 15 mg per 100 cm³. The LOD and LOQ values for DFB were 0.142 to 0.432 respectively. When various technical and formulated smples were analysed by this proposed method the percentage recoveries were found to be 99.20-101.80% with RSD between 0.01% to 0.45%.

Published in	Science Journal of Energy Engineering (Volume 5, Issue 6)
DOI	10.11648/j.sjee.20170506.14
Page(s)	152-157
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Diflubenzuron, Stability Indicating HPLC, Diphenyl Internal Standard

References

[1]	‘Diflubenzuron’, Metabolic Pathways of Agrochemicals, Part-II (Insecticides and Pesticides), Editor- T. Roberts and D. Hutson, RSC publications 1999, 344.
[2]	Mensah J K, Lundanes E, Grebrokk T, Holen B, J. Chromatogr. A, 21 Mar, 1997, 765(s1), 85-90.
[3]	Nigg H N, Cannizzaro R D, Stamper J H, Bull. Environ. Contam. Toxicol., Jun 1986, 36(6), 833-838.
[4]	Wimmer M J, Smith R R, Jones J P, J. Agric. Food Chem., Feb 1991, 39(2), 280-286.
[5]	Rodriguez E, Barrio R J, Goicolea A, Gomez de Balugera Z, Anal. Chim. Acta, 22 Mar 1999 384 (1), 63-70.
[6]	Rodriguez E, Gomez de Balugera Z, Guicolea M A, Barrio R J, J. Liq. Chromatogr. Relat. Technol., Jul 1998, 21 (12)' 1857-1870.
[7]	Gao B F, Jin X H, Fang C G, Fang Q X, Zhang Y Q, Yu W B, Fenxi Huaxue, Jul 1996, 24(7), 865(Chinese).
[8]	Bicchi C, Balbo C, Binello A, J. High Resolut. Chromatogr., Feb 1996, 19(2), 105-110.
[9]	Terashi A, Eto S, Takano K, Shokunin Eiseigaku Zasshi, Apr 1993, 34(2), 114-119 (Japanease).
[10]	Sundaram K M S, Nott R, J. Liq. Chromatogr. 1989, 12(12), 2333-2343.
[11]	Bicchi C, Balbo C, D' Amato A, Panero O Chromatographia, Oct 1996, 43 (7-8).
[12]	Brede C, Lundanes E. J. Chromatogr. A, 29 Sep 1995, 712(1), 95-101.
[13]	Knowles D E, Richter B E, Anderson M R, Later D W. Chim. Oggi., Jan-Feb 1989, 7 (1-2), 11-15.
[14]	Sherma J., Rolfe C. J Chromatogr., 23 Jul 1993, 643(1-2), 337-339.
[15]	CIPAC Handbook Volume H, ‘Analysis of technical and formulated pesticides’, Editor-W Dorbat and A Martin, CIPAC Limited, 1998, pp. 141-146.

Cite This Article

Plain Text BibTeX RIS

APA Style

Vinayak Ambike, Anant Argekar. (2017). Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide. Science Journal of Energy Engineering, 5(6), 152-157. https://doi.org/10.11648/j.sjee.20170506.14

Copy | Download

ACS Style

Vinayak Ambike; Anant Argekar. Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide. Sci. J. Energy Eng. 2017, 5(6), 152-157. doi: 10.11648/j.sjee.20170506.14

Copy | Download

AMA Style

Vinayak Ambike, Anant Argekar. Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide. Sci J Energy Eng. 2017;5(6):152-157. doi: 10.11648/j.sjee.20170506.14

Copy | Download

@article{10.11648/j.sjee.20170506.14,
  author = {Vinayak Ambike and Anant Argekar},
  title = {Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide},
  journal = {Science Journal of Energy Engineering},
  volume = {5},
  number = {6},
  pages = {152-157},
  doi = {10.11648/j.sjee.20170506.14},
  url = {https://doi.org/10.11648/j.sjee.20170506.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjee.20170506.14},
  abstract = {A new, fast, accurate, reliable and stability indicating HPLC method for the determination of Diflurobenzuron (DFB), in technical and formulation samples in presence of related and degraded impurities has been developed using intersil – 3 stainless steel C. 18 column (5 µm, 250 mm length x 4.6 mm id), acetonitrite: water: 1.4 dioxane (58:39:0.03 v/v) as mobile phase and diphenyl as internal standard. The flow rate of the mobile phase was 2 cm3 min-1. Detection was carried out at 260 mm using UV detector. The retention times were 1.55 min, 1.75 min, 6.3 min, 7.9 min and 10.7 min for 2, 6 diflurobenzoic acid, 2, 6 diflurobuzamide, diflurobenzuron, difur (an impurity) and diphenyl (internal standard) respectively. The linearity range of DFB was 0.5 to 15 mg per 100 cm3. The LOD and LOQ values for DFB were 0.142 to 0.432 respectively. When various technical and formulated smples were analysed by this proposed method the percentage recoveries were found to be 99.20-101.80% with RSD between 0.01% to 0.45%.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide
AU  - Vinayak Ambike
AU  - Anant Argekar
Y1  - 2017/12/01
PY  - 2017
N1  - https://doi.org/10.11648/j.sjee.20170506.14
DO  - 10.11648/j.sjee.20170506.14
T2  - Science Journal of Energy Engineering
JF  - Science Journal of Energy Engineering
JO  - Science Journal of Energy Engineering
SP  - 152
EP  - 157
PB  - Science Publishing Group
SN  - 2376-8126
UR  - https://doi.org/10.11648/j.sjee.20170506.14
AB  - A new, fast, accurate, reliable and stability indicating HPLC method for the determination of Diflurobenzuron (DFB), in technical and formulation samples in presence of related and degraded impurities has been developed using intersil – 3 stainless steel C. 18 column (5 µm, 250 mm length x 4.6 mm id), acetonitrite: water: 1.4 dioxane (58:39:0.03 v/v) as mobile phase and diphenyl as internal standard. The flow rate of the mobile phase was 2 cm3 min-1. Detection was carried out at 260 mm using UV detector. The retention times were 1.55 min, 1.75 min, 6.3 min, 7.9 min and 10.7 min for 2, 6 diflurobenzoic acid, 2, 6 diflurobuzamide, diflurobenzuron, difur (an impurity) and diphenyl (internal standard) respectively. The linearity range of DFB was 0.5 to 15 mg per 100 cm3. The LOD and LOQ values for DFB were 0.142 to 0.432 respectively. When various technical and formulated smples were analysed by this proposed method the percentage recoveries were found to be 99.20-101.80% with RSD between 0.01% to 0.45%.
VL  - 5
IS  - 6
ER  -

Copy | Download

Author Information

Vinayak Ambike

Department of Chemistry, The Institute of Science, Mumbai, India
Anant Argekar

Department of Chemistry, The Institute of Science, Mumbai, India

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Vinayak Ambike, Anant Argekar. (2017). Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide. Science Journal of Energy Engineering, 5(6), 152-157. https://doi.org/10.11648/j.sjee.20170506.14

Copy | Download

ACS Style

Vinayak Ambike; Anant Argekar. Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide. Sci. J. Energy Eng. 2017, 5(6), 152-157. doi: 10.11648/j.sjee.20170506.14

Copy | Download

AMA Style

Vinayak Ambike, Anant Argekar. Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide. Sci J Energy Eng. 2017;5(6):152-157. doi: 10.11648/j.sjee.20170506.14

Copy | Download

@article{10.11648/j.sjee.20170506.14,
  author = {Vinayak Ambike and Anant Argekar},
  title = {Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide},
  journal = {Science Journal of Energy Engineering},
  volume = {5},
  number = {6},
  pages = {152-157},
  doi = {10.11648/j.sjee.20170506.14},
  url = {https://doi.org/10.11648/j.sjee.20170506.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjee.20170506.14},
  abstract = {A new, fast, accurate, reliable and stability indicating HPLC method for the determination of Diflurobenzuron (DFB), in technical and formulation samples in presence of related and degraded impurities has been developed using intersil – 3 stainless steel C. 18 column (5 µm, 250 mm length x 4.6 mm id), acetonitrite: water: 1.4 dioxane (58:39:0.03 v/v) as mobile phase and diphenyl as internal standard. The flow rate of the mobile phase was 2 cm3 min-1. Detection was carried out at 260 mm using UV detector. The retention times were 1.55 min, 1.75 min, 6.3 min, 7.9 min and 10.7 min for 2, 6 diflurobenzoic acid, 2, 6 diflurobuzamide, diflurobenzuron, difur (an impurity) and diphenyl (internal standard) respectively. The linearity range of DFB was 0.5 to 15 mg per 100 cm3. The LOD and LOQ values for DFB were 0.142 to 0.432 respectively. When various technical and formulated smples were analysed by this proposed method the percentage recoveries were found to be 99.20-101.80% with RSD between 0.01% to 0.45%.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide
AU  - Vinayak Ambike
AU  - Anant Argekar
Y1  - 2017/12/01
PY  - 2017
N1  - https://doi.org/10.11648/j.sjee.20170506.14
DO  - 10.11648/j.sjee.20170506.14
T2  - Science Journal of Energy Engineering
JF  - Science Journal of Energy Engineering
JO  - Science Journal of Energy Engineering
SP  - 152
EP  - 157
PB  - Science Publishing Group
SN  - 2376-8126
UR  - https://doi.org/10.11648/j.sjee.20170506.14
AB  - A new, fast, accurate, reliable and stability indicating HPLC method for the determination of Diflurobenzuron (DFB), in technical and formulation samples in presence of related and degraded impurities has been developed using intersil – 3 stainless steel C. 18 column (5 µm, 250 mm length x 4.6 mm id), acetonitrite: water: 1.4 dioxane (58:39:0.03 v/v) as mobile phase and diphenyl as internal standard. The flow rate of the mobile phase was 2 cm3 min-1. Detection was carried out at 260 mm using UV detector. The retention times were 1.55 min, 1.75 min, 6.3 min, 7.9 min and 10.7 min for 2, 6 diflurobenzoic acid, 2, 6 diflurobuzamide, diflurobenzuron, difur (an impurity) and diphenyl (internal standard) respectively. The linearity range of DFB was 0.5 to 15 mg per 100 cm3. The LOD and LOQ values for DFB were 0.142 to 0.432 respectively. When various technical and formulated smples were analysed by this proposed method the percentage recoveries were found to be 99.20-101.80% with RSD between 0.01% to 0.45%.
VL  - 5
IS  - 6
ER  -

Copy | Download